Antibiotic resistance of Klebsiella pneumoniae isolated from samples (blood, sputum, surgical wound, urine) taken from resuscitation patients

Antimicrobial resistance represents a global problem and remains a major challenge in the field of healthcare. This is a natural process and it is facilitated by the misuse of antibiotics. The resistance problem towards antibiotics is not new. It appeared, exactly on that day, when the first antibiotic was discovered. Alexander Fleming himself noticed shortly after the discovery of penicillin that some microorganisms, which were initially affected by the antibiotic became insensitive to it. In 1946, 6% of the microbes prevalent at the time were resistant to penicillin, and in 1964, it was already 60%, that were resistant to Penicillin. Our goal is to study the resistance of Klebsiella pneumoniae to antimicrobial drugs. Bacterial strains are separated out from the samples taken from resuscitation patients, indicating that we are dealing with clinical strains the resistance of which is always different from that of wild strains. Since 2018, in accordance with the instruction of WHO, collecting and sending data on antibiotic susceptibility test results of microorganisms Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Streptococcus pneumoniae, Acinetobacter baumanii/calcoaceticus, Enterococcus faecalis/faecium separated out from bacteriological laboratories throughout Georgia, is carried out by General Bacteriology Laboratory of Lugar Center for Public Health Research of National Center for Disease Control and Public Health. We got interested in Klebsiella pneumonia, which belongs to one of the most prevalent pathogens group of clinical significance. The study of susceptibility to antimicrobial drugs is being carried out in agar by diffusion method. The Keurby-Bauer Antibiotic Disc Test and the E-Test impregnated strips with different concentrations of antibiotic for the determination of the minimum inhibitory concentration were used. Susceptibility to antimicrobial drugs is studied and interpreted according to the European standard EUCAST.