The identification and study of the endogenous growth inhibitory factor isolated from benign tumor cells of the human prostate

By the method of alcohol extraction, the thermostable protein complex was identified and isolated from the postoperative material of patients with benign prostatic hyperplasia. A comparative study of the protein components of this complex has shown that similar to the protein complexes isolated from the cells of the other eukaryotic organisms, the complex derived from the benign prostate tumor cells contains two sub-fractions of proteins: the relatively high molecular weight 20-120 kDa, and the low molecular weight 12-17 kDa. At the same time, the electrophoresis in the polyacrylamide gel showed that the TPC of benign prostate cancer cells revealed a minor content of the active component (calmodulin, mp 12-14 kDa). Experiments with anti-calmodulin antibodies proves that small amounts of calmodulin are typical for benign tumor cells in the human prostate. The inhibitory effect of thermo-stable protein complex isolated from benign tumor cells of human prostate cells on the proliferative activity of various (liver and brain) tissues of adolescent rats has not been revealed.